Gene expression study related with the intrinsic pathway of apoptosis in bladder cancer by real-time PCR technique
Barione DF, Lizarte FS, Novais PC, de Carvalho CA, Valeri FC, Peria FM, de Oliveira HF, Zanette DL, Silva WA Jr, Cologna AJ, Reis RB, Tucci S Jr, Martins AC, Tirapelli DP, Tirapelli LF.Genet Mol Res. 2013 Apr 2;12(2):878-86. doi: 10.4238/2013.April.2.4.

Source

Departamento de Cirurgia e Anatomia, Faculdade de Medicina de RibeirãoPreto, Universidade de São Paulo, RibeirãoPreto, SP, Brasil.

Abstract

We examined the expression of anti-apoptotic genes (XIAP and Bcl-2) and apoptotic genes (cytochrome c, caspase-9, Apaf-1) in tissue samples of patients with superficial bladder cancer. Thirty-two bladder cancer tissue samples (8 papillary urothelial neoplasm of low malignant potential, 10 low-grade, and 14 high-grade) and 8 normal bladder tissue samples from necropsy were used for the study of gene expression by real-time PCR analysis. Analysis of the expression of apoptotic gene constituents of an apoptosome demonstrated an increase in Apaf-1 expression in the three tumor grades when compared with the control (P < 0.01, P < 0.05, and P < 0.01), low expression of caspase-9 in all groups (P < 0.05), and an increase in cytochrome c expression in all tumor grades in relation to the control, although without statistically significant difference. The expression of anti-apoptotic genes revealed an increase in XIAP expression in all tumor grades in relation to the control, although without statistically significant difference, and low expression of Bcl-2 in all tumor grades and the control (P < 0.05). The results proved that there is low evidence of apoptotic activity by the intrinsic pathway, demonstrated by the low expression of caspase-9 and considerable increase in XIAP expression, which may render these genes potential therapeutic targets in bladder cancer treatment.