Adult testosterone and calculated free testosterone reference ranges by tandem mass spectrometry
Neale SM, Hocking R, Biswas M, Turkes A, Rees D, Rees DA, Evans C.Ann ClinBiochem. 2013 Feb 12. [Epub ahead of print]

Source

Department of Medical Biochemistry & Immunology, University Hospital of Wales, Heath Park.

Abstract

BACKGROUND:

Testosterone is measured for the investigation of female hyperandrogenism and male hypogonadism. Liquid chromatography-tandem mass spectrometry (tandem MS) is becoming the method of choice but comprehensive reference ranges are lacking.

METHODS:

Testosterone was measured by tandem MS on 90 healthy women, 67 young healthy men and pregnant women (59 first trimester and 60 second trimester).

RESULTS:

The male, male calculated free, first trimester and second trimester testosterone reference ranges (derived using the antilog of mean ± 1.96 SD of log transformed data) were 10.6-31.9, 0.23-0.63, 0.6-4.9 and 0.9-4.9 nmol/L, respectively. The female testosterone upper reference range limit, derived non-parametrically from the 97.5th centile, was <1.7 nmol/L.

CONCLUSIONS:

We have derived tandem MS testosterone reference ranges to support clinical services.