Adult testosterone and calculated free testosterone reference ranges by tandem mass spectrometry
Neale SM, Hocking R, Biswas M, Turkes A, Rees D, Rees DA, Evans C.Ann ClinBiochem. 2013 Feb 12. [Epub ahead of print]


Department of Medical Biochemistry & Immunology, University Hospital of Wales, Heath Park.



Testosterone is measured for the investigation of female hyperandrogenism and male hypogonadism. Liquid chromatography-tandem mass spectrometry (tandem MS) is becoming the method of choice but comprehensive reference ranges are lacking.


Testosterone was measured by tandem MS on 90 healthy women, 67 young healthy men and pregnant women (59 first trimester and 60 second trimester).


The male, male calculated free, first trimester and second trimester testosterone reference ranges (derived using the antilog of mean ± 1.96 SD of log transformed data) were 10.6-31.9, 0.23-0.63, 0.6-4.9 and 0.9-4.9 nmol/L, respectively. The female testosterone upper reference range limit, derived non-parametrically from the 97.5th centile, was <1.7 nmol/L.


We have derived tandem MS testosterone reference ranges to support clinical services.